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Phospholipid profiling revealed that arachidonic acid is preferentially incorporated into phosphatidylethanolamine (PE) and phosphatidylcholine (PC) species containing C16 and C18 acyl chains in an ACSL4-dependent manner (77)

The solution was then transferred to a Teflon lined stainless steel autoclave (Parr acid digestion vessel, 45 mL) and heated in an oven to 180 C for 20 h