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doi: 10.1038/s41598-021-85673-w 68 SokoloffL.ReivichM.KennedyC.RosiersM

"Anemia: Practice Essentials, Pathophysiology, Etiology"

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Primary normal (from donor young and old) and IPF fibroblasts were isolated as previously described (9), cultured in Hams F12 medium (Gibco) with 10% FBS (Gibco) and 1% of antimycotic-antibiotic (Gibco), and used in passages 4 to 6

Antibiotics can alter the gut microbiota, and microbes can also modify the drugs, altering their chemistry and leading to the development of drug resistance

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