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Tissue staining Five-micron formalin-fixed, paraffin-embedded tissue sections were used for hematoxylin and eosin (H&E) staining immunohistochemistry and immunofluorescence analyses

Amino acid metabolism

Abbreviations AMPK: AMP-activated protein kinase ARE: Antioxidant response element CBS: Cystathionine beta-synthase CSE: Cystathionase CYST: Cystathionine DCFDA: Dichlorofluoresin diacetate ECAR: Extracellular acidification rate ESI: Electrospray ionization ETC: Electron Transport Chain FCCP: Carbonyl cyanide p-trifluoro-methoxyphenyl hydrazone GSH: Glutathione GSSG: Oxidized glutathione HCY: Homocysteine MAT2A: Methionine adenosyltransferase MTT: 3-(4, 5-Dimethyl-2-tetrazolyl)-2,5-diphenyl-2H tetrazolium bromide assay NAC: N-acetylcysteine Nrf-2: Nuclear factor erythroid-derived 2-like OCR: Oxygen consumption rate OXPHOS: Oxidative phosphorylation ROS: Reactive oxygen species SAM: S-adenosylhomocytseine References Rawla P (2019) Epidemiology of prostate cancer, world