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CRISPR-mediated GPX4 deletion Guide RNAs targeting the first exon of human GPX4 were designed using the CRISPR Design tool (Zhang lab, MIT)

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Furthermore, MGL exerted its protective effects by inhibiting the PI3K/AKT signaling pathway [492] and the arachidonate 5-lipoxygenase (ALOX5)-mediated ferroptosis, inhibiting M1 while promoting M2 macrophages [493]

Analysis of ALT (alanine aminotransferase, a serum marker of liver damage) revealed a strong increase in Atp7b / mice, while Atp7b / :Prnp / animals exhibited significantly lower ALT values (Fig