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Ugalde et al ., 2022 a ) were amplified using PCR (Phusion High Fidelity DNA Polymerase, Thermo Fisher Scientific): The AP1 sp was amplified, adding an attB1 site and an overlap to roGFP2 using the primer B1_AP1sp_F GGGGACAAGTTTGTACAAAAAAGCAGGCTTAatgggggcatcgaggagt and AP1sp_ro2Orp_P2 GCCCTTGCTCACCATgcgagggcttgcctcagc

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