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In vitro, NRF2 overexpression in murine HCC cells activated the COX2/PGE2/EP4 pathway and upregulated TGF and VEGF-A

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The reverse pathway is present in several organisms, including humans, and involves the transfer of the thiol group from homocysteine to cysteine via a similar mechanism

Cells were collected via dissociation by TrypLE Express and pelleted by centrifugation (300 x g , 3 min, room temperature in 15-ml conical tubes, followed by 21,000 x g , 2 min, room temperature in microcentrifuge tubes) and stored at -80C until further analysis