hPSCs were dissociated into single-cell suspensions using TrypLE Select (Thermo Fisher Scientific, Netherlands) and plated at a density of 2 10 6 cells/well in Stem Flex medium (Thermo Fisher Scientific, Netherlands) supplemented with 10 M Y-27632 (Peprotech, USA) on Geltrex-coated 6-well plates (Eppendorf, Germany) under a 5% CO 2 atmosphere

Photo protocol When to take photos: Day 1 before starting Every 2 weeks (same day, same time) Always in morning, before eating, after bathroom How to take photos: Same lighting every time (natural window light ideal) Same location, same distance from camera Same poses: front relaxed, front flexed, side relaxed, side flexed, back Wear minimal clothing (underwear works) No filters, no editing Common mistakes: Inconsistent lighting (biggest factor in bad comparisons) Different times of day (water retention varies) Flexing in "after" but not "before" Cherry-picking best angles Measurements matter more than scale Track these every 2 weeks: Body weight (same time, same conditions) Waist circumference (at navel) Chest, arms, thighs (if building muscle) Body fat percentage (calipers or DEXA) Photos + measurements + strength progression = complete picture
L-glutathione, on the other hand, is also an antioxidant that could inhibit tyrosinase step in the pathogenesis of pigmentation
A randomized, double-blind, placebo-controlled trial of duloxetine in the treatment of women with fibromyalgia with or without major depressive disorder
BMJ 319, 807811
Twice-daily dosing (500mcg morning and evening) shows up in more aggressive protocols but adds cost and injection logistics on top of an already-active TRT schedule