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RNA ISH was performed as previously described ( Nuclear and Cytoplasmic RNA Fractionation Analysis Nuclear and cytoplasmic RNA isolation in the mle-12 cells was performed using the Cytoplasmic and Nuclear RNA Purification Kit (Norgen, Belmont, CA, United States) according to the manufacturers instruction

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These biomarkers not only act as indirect indicators of resolution potential but also provide tools to evaluate disease progression, predict therapeutic responses, and guide indication selection and target identification for PRLM-based therapies [29]

[DOI] [PMC free article] [PubMed] [Google Scholar] 53.Tak LM, Cleare AJ, Ormel J, Manoharan A, Kok IC, Wessely S, et al

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A complementary approach is to develop a therapeutic reagent to treat the root of these diseases, dubbed geroscience