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Given the inverse relationship between GSH levels and tumor sensitivity to chemotherapy, it is plausible that Curcumin-induced GSH depletion can enhance the effectiveness of Cisplatin-based treatment for EC

2) Add 60 l of Buffffer solution to each well

However, knowing this also lets you attack the problem

Sci Rep 12(1):8042

In summary, the GSH-GSSG redox cycle does not exist in isolation, but rather operates as a dynamic equilibrium system that is highly coupled to the NADPH metabolic network