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100% EtOH used as solvent for drug A 0.1M phosphate buffer pH 6.7, 1mM CDNB and GSH as substrates B 90 second preincubation of drug and enzyme prior to start of reaction C 10x dilution of 1-chloro 2,4-dinitrobenzene (CDNB) (0.1mM), and glutathione (GSH) (0.1mM) in the assay solution D GSH affinity purified mouse liver cytosol extract NA = No replicate assays performed Effect of Atazanavir on Bilirubin Binding to GSTs For the alpha class GSTs (hGSTA1-1 and hGSTA2-2) a characteristic biphasic CD spectrum with overlapping bands of opposite sign were observed after binding of stoichiometric amounts (one-per subunit) of bilirubin to the protein (Figure 2A)

ZhuGYangSXieZWanX.Synaptic modification by L-theanine, a natural constituent in green tea, rescues the impairment of hippocampal long-term potentiation and memory in AD mice

10.1016/j.bbrc.2018.11.124 Biochem Biophys Res Commun

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Huang X, El-Sayed MA

doi: 10.1186/1471-2369-13-92