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2.1.2 The role of Cys93 in counteracting tissue hypoxia Among the conserved amino acids in hemoglobin, the cysteine at position 93 on the -globin chain (Cys93) plays a key role in the physiological response to low oxygen availability ( During hypoxic stress, hemoglobin undergoes a conformational shift that exposes Cys93, allowing SNO-hemoglobin (SNO-Hb) to form

After adjusting the pH value and fixing the volume, the assay was conducted