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Briefly, after drug treatment, cells were harvested and washed twice with cold PBS and then stained with annexin-V for 15 min at room temperature in the dark, followed by staining with PI

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Additionally, TFRD reduced MDA and ROS levels in the serum and the expression of Notch1, Hes1 and PRDX1 proteins in femur tissues, suggesting that TFRD plays an anti-osteoporosis role by inhibiting the activation of the Notch1/Hes1/PRDX1 signalling pathway, alleviating oxidative stress injury, inhibiting osteoclastogenesis and bone resorption, promoting bone formation and regulating bone homeostasis

The therapeutic efficacy of Lorazepam in preserving spinal cord integrity underscores the clinical potential of targeting pH-sensing GPCRs in ischemia-reperfusion pathologies