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Cell Culture and in vitro Model of Hemorrhagic Toxicity Astrocytes and 293T cells (Chinese Academy of Sciences, Shanghai) were cultured in dulbeccos modified eagle medium (DMEM) (Gibco, UA) added 10% fetal bovine serum (Gibco, Australia), following which they were incubated at 100% humidity in an environment containing 5% CO 2

Beyond its established roles in apoptosis, p53 can also modulate ferroptosis through metabolic and redox regulatory programs

doi: 10.1016/j.cytogfr.2023.07.005, 52

Anti-MuRF1 (1:1000, GTX110475) was purchased from GeneTex (Irvine, CA, USA)

According to recommendations from nutrition institutes and the World Health Organization (WHO), the daily Recommended Dietary Allowance (RDA) is as follows: Infants 06 months: 0.4 mcg Infants 712 months: 0.5 mcg Children 13 years: 0.9 mcg Children 48 years: 1.2 mcg Children 913 years: 1.8 mcg Teenagers 14+ years and adults: 2.4 mcg Pregnant women: 2.6 mcg Breastfeeding women: 2.8 mcg Receiving adequate daily Vitamin B12 helps reduce the risk of anemia, fatigue, and neurological problems, while supporting optimal metabolic and overall bodily function

Endogenous compounds, including signaling molecules derived from arachidonic acid and associated enzymes, have the ability to bind and stimulate endogenous cannabinoid receptors, leading to elevated quantities of RNA and protein [104]