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Other assessed sleep dimensions include: sleep insufficiency, assessed using the item do you consider that you get enough sleep, rated on 5-point scale from 0 yes, definitely enough to 4 No, definitely not enough

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The resulting supernatant was then filtered twice through centrifugal filters (0.22 m, hydrophilic PVDF, 0.5 mL, Millipore) at 2000 g for 5 minutes each

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