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564,565 Clinical strategies targeting redox signaling in disease treatment Oxidative stress plays a significant role in the onset and progression of various diseases

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To measure complex-specific H 2 O 2 production, the cells were incubated with 10 mM pyruvate/1 mM malate, 10 mM succinate/2 M rotenone, or 5 mM glycrol-3-phosphate/2 M rotenone diluted in MAS buffer containing 4 mM ADP and 1 nM XF PMP for 30 min at 37 C prior to the addition of Amplex Red

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Beyond these direct effects on redox status, Nrf2 also modulates the expression of genes implicated in cellular defense, thereby inhibiting redox cycle reactions and diminishing GSH consumption (52)