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The results indicated a significant upregulation in the mRNA and protein expression levels of HK2 following I/R treatment

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2.10 Cell lines and cell culture This study used the human epidermoid carcinoma cell line KB-3-1 as the drug-sensitive cell line, and its MRP1-overexpressing cell line KB/CV60 (Supplementary Figure S1A), which was maintained in the medium with 1 g/mL of cepharanthine and 60 ng/mL of vincristine (Supplementary Figure S1B) by transfecting recombinant pcDNA3.1/MRP7 plasmids and the parental human ovarian adenocarcinoma cell line SKOV3 (Wang et al., 2021b) were also used