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The entire length of the needle, up to the hub, should be inserted into the muscle Attach your syringe and pull back on the plunger (draw back) to check for blood If blood is seen in the syringe: Detach your syringe Pull needle half way out Change direction of the needle by a few degrees Re-insert needle Draw back again to check theres no blood If no blood is seen inject medication slowly over a few seconds Once syringe is empty the needle and syringe should be removed together in one smooth movement Disposal of equipment Needles and syringes should be stored somewhere safe e.g

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To evaluate whether group differences in histological outcomes remained significant when accounting for subject- or animal-level variability, we reanalyzed all relevant datasets using linear mixed-effects models