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ROS and lipid peroxidation assay Day 12 cultured SCD erythroblasts under different treatments were incubated with 5 M 2-7-dichlorodihydrofluorescein diacetate (H2DCFDA) (#D6883, Sigma) for 30 min at 37 C, washed with PBS, and analyzed on a BD LSR-II flow cytometer for ROS level

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Thus, modifying the known binding sites on tRNA could be advantageous in altering interactions between tRNA and specific components during the protein expression process

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Free radicals produced by ROS direct undesirable changes and result from lipid peroxidation, DNA fragmentation, cell death, DNA damage, protein modification, and membrane damage

4.4.1.6 Oxidized low-density lipoprotein oxLDL is generated when LDLs lipid core and apoB are oxidatively modified, yielding pro-inflammatory particles that impair endothelial function