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Description

Dmrta2 +/ and Dmrta2 Tg/+ (De Clercq et al., 2018), Pax6 sey/+ (Hill et al., 1991), and BAT-gal (Maretto et al., 2003) mice were maintained in a C57Bl/6 background

On the basis of the determined protein concentrations, an appropriate volume of loading buffer was added, and the samples were heated in a liquid metal bath to denature the proteins