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There have been fairly large studies in recent years that examined exactly this issue

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As for the blank solution, 2.5 mL of unitary phosphate buffer (PB) solution was used, while a solution mixture (1.45 mL of PB + 50 L of H 2 O + 1 mL of diluted H 2 O 2 ) was taken as a reference analyte, and in the test sample (1.45 mL of PB + 50 L of plasma sample + 1 mL of diluted H 2 O 2 ) was used