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Briefly, 2030 oocytes were lysed on ice in mammalian lysis buffer 5X (ab179835, Abcam), then deproteinized, neutralized, and diluted to concentrations suitable for standard curve fitting

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Nanopore sequencing also comes up with a few disadvantages : limited discrimination between heterozygous and homozygous alleles [98], long and rare methylation motifs can be missed by nanopore sequencing [99], the need for high-quality DNA of high molecular weight (requiring invasive techniques for extraction from blood or other tissues) [52]

A balanced cortisol response is needed to restore optimal nutrient absorption, hormone balance, and sled-wake cycles