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10.1038/srep42851 66

Right after, animals were washed twice with M9 buffer, plated to RNAi plates, and grown for 16 h

The primer sequences are listed in Table S1

* And heres the kickercurrent research suggests these declines are sharper and more widespread in modern men than at any other point in recorded history [1,2]

A Chemi-Doc imager was used to acquire the band intensities after applying the Western Lightning Plus ECL Chemiluminescence substrates (Perkin Elmer, USA)