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Environ Sci Pollut Res 16:795804 Sengupta D, Ramesh G, Mudalkar S, Kumar KRR, Kirti PB, Reddy AR (2012) Molecular cloning and characterization of -Glutamyl cysteine Synthetase (VrECS) from roots of Vigna radiata (L.) Wilczek under progressive drought stress and recovery

0.3 million cells per sample were incubated with the complex in wash buffer (PBS with 2% BSA) for 30 min at 4 C followed by incubating with the detection antibody (Supplementary Table 2) for another 30 min at 4 C in the dark after washing the excess amount of the complex

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doi: 10.1016/j.npep.2015.09.005

Intervention effects of okra extract on brain-gut peptides and intestinal microorganisms in sleep deprivation rats