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This means that when looking for toxicants in human brains, large numbers of participants are required, since the relevant toxicant may only be observable if the toxicant exposure was recent or ongoing

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The peroxidase-conjugated goat anti-rabbit (Sigma-Aldrich, A6154) or peroxidase-conjugated goat anti-mouse (Sigma-Aldrich, A4416) second antibodies were diluted 1:10,000 and incubated 1 h at RT