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Molecules (2023) 28:3389

Immunoblot analysis Whole cell lysates were prepared using radio-immunoprecipitation assay (RIPA) lysis buffer (pH 7.8) containing 25 mM Tris-HCl, 120 mM NaCl, 5 mM EDTA and 0.5% NP-40 supplemented with 100 g/mL phenylcmethylsulfonyl fluoride, 200 mM sodium orthovanadate, 0.5 M NaF, protease inhibitor cocktail sets III and IV and phosphatase inhibitor cocktails 2 and 3 (Sigma-Aldrich)

Abnormal iron metabolism, oxidative stress, and pathways related to ferroptosis have been well-marked to promote cellular damage in RM

This shows the parts of a Luer Slip Syringe used for giving injections Steps of Giving an Injection Always use a clean needle and syringe Wash your hands with antiseptic soap before handling the needle and syringe

[Abstract] Immun Inflamm Dis

Stool samples can also be used to find signs of infection in the digestive tract