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Cells were pre-treated with 10 mM NAC in media for 12 h to supplement the cellular glutathione concentration

This feature has an extinction coefficient of 1000 M 1 cm 1 and is assigned as charge transfer arising from the proximity of the NADPH dihydronicotinamide ring and the flavin isoalloxazine

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Eluted peptides were analysed by the MS in positive polarity using a data-independent acquisition mode as follows: an initial MS1 scan was carried out at 140,000 resolution with an automatic gain control target of 3 10 6 for a maximum injection time of 200 ms, m / z range: 4101,600

Arch Physiol Biochem