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To induce GPX2 overexpression, cells were treated with 1 g/mL doxycycline for 24 h

Meyers, L

The expression of adipogenic genes is decreased in obesity and diabetes mellitus

This study was supported by the Anhui Traditional Chinese Medicine Inheritance and Innovation Research Project: 2024CCCX004, the Anhui Huatuo Institute of Traditional Chinese Medicine Science and Technology Major Project Unveiled List Project: BZKZ2402, the Anhui Provincial Natural Science Foundation Project: 2208085MH273, and the Anhui Provincial Key Research and Development Program: No.202104j07020012, Scientific Research Fund Project of Anhui University of Traditional Chinese Medicine: 2022ZJPT01 and Anhui Provincial Medical and Health Key Specialty Construction Project: Anhui Health Science and Education (2021) No.273

These mice were perfused with PBS at 0.5, 2, 6, 12, 24 h after the last treatment ( n = 3 per group), and their brains were collected and homogenized in the buffer containing 10 mM HEPES, 1.5 mM MgCl 2 , 10 mM KCl, 100 mM DTT, and 1 mM PMSF (pH 7.9) (5 ml/gram of brain) by sonication on ice (one pulse of 0.75 s for 2 min, UP200H, Hielscher, USA)

Interestingly, these results were not always consistent