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For lipid reporter experiments, HEK293 cells were co-transfected with plasmids with AKT-PH::GFP and shRNA against KIAA1109 (Sigma-Aldrich, TRCN0000263343 with 73% knockdown efficiency), incubated for 48 h and imaged by confocal microscopy for membrane localized GFP

This review summarizes the mechanisms by which MPs exposure induces gut microbiota dysbiosis and skin damage from an integrated gutskin axis perspective, highlighting their interplays relevance

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The refinement was carried out in several cycles of automated refinement in phenix.refine program [43] and manual model building performed in Coot [44]

Methods: Male Wistar rats were subjected to a chow (CHOW) diet or a free-choice (fc) diet of either chow, saturated fat and liquid sugar (fcHFHS), chow and saturated fat (fcHF), or chow and liquid sugar (fcHS) for 4 weeks