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Immediately prior to analysis, cells were brought to room temperature, washed 2x with 1.5 mL cold HBSS, and stained with solution containing RNase A solution (1:100 v/v, Cat# 19101, Qiagen, Hilden, Germany) at and propidium iodide (PI) solution (1:20 v/v, Cat# P3566, Thermo Fisher Scientific) for 30 min at 37C

Possible side effects: Rare allergic reactions

Based on structural and functional differences, the family is divided into two groups: Rel proteins (RelA, RelB, and c-Rel), which possess transcription activation domains (TADs), and NF-B1 and NF-B2, which lack TADs in their mature forms (p50 and p52) and require co-activators such as Bcl-3 for transcriptional activity [6]

A precursor for glutamate called alpha-ketoglutarate is created in the mitochondria in the TCA cycle (Krebs cycle, producing ATP energy)

Investigators: Craig B