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Oxidative stress markers were significantly altered, demonstrating hypoxia-induced hepatic oxidative stress

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Afterwards, a watershed segmentation algorithm was applied to the 3D cropped stack (Step 3), and the slice with the largest segmented area along z-axis (Midplane slice, Step 3 middle image with red edge) was selected to generate a membrane contour (white outline in Step 4) for quantifying GUV radius and ALPS 1-2-eGFP binding intensity

Interestingly, a role for UCP2 in the transport of mitochondrial glutathione has been reported in neurons (de Bilbao et al., Mitochondria contain a myriad of antioxidant defense enzymes and low molecular weight molecules that degrade both O 2 and H 2 O 2 with exquisite efficiency (Mailloux et al., 2013a)