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Interestingly, the effects of autophagy gene knockout differed based on the genetic background, especially regarding crucial tumor suppressors like APC and Tp53

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This leads to an impaired conversion of primary to secondary BAs, markedly shifting the overall BA composition

Instead, asparagine supplementation enhances the expression of glutamine synthetase (GLUL) and increases intracellular glutamine usage via glutaminolysis, resulting in the recovery of global protein translation that is blocked by glutamine deprivation 45

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In this review, we characterize glutamine metabolism in cancer cells and explore the specific mechanisms by which glutamine affects cancer cell proliferation, apoptosis, invasion and metastasis, and tumor immune escape