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One approach in creating a mitochondria-targeted AT is to bind an antioxidant molecule (e.g., SOD, thiol, vitamin E, BHT, ebselen, CoQ, or various spin traps, such as TEMPO) with a hydrocarbon chain of varying length to the phosphorus of the lipophilic compound triphenylphosphonium (TPP) such as mitoquinol (MitoQ 10

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Factors that contribute to these variations include an individual's baseline glutathione levels, skin type, lifestyle, the specific skin concerns being addressed, and the consistency of treatments

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Cloning the hGrx1-roGFP2 constructs The hGrx1-roGFP2 coding sequence [69] was amplified using the forward (Grx1 primer: 5 - AGTCGGTACCATGGCTCAAGAGTTTGTGAACT - 3) and reverse (roGFP2 primer: 5- AACCCCCGGGTTACTTGTACAGCTCGTCCATG -3) primers and cloned into the pARL+ expression vector [34], using Kpn I and Xma I restriction sites (underlined)