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250 l of Passive Lysis buffer (Promega) was added to each well of the 12well plate, 48 h posttransfection

The inflammatory influx of B and T cells suggests that acute immune attacks are dominantly driven by T cell infiltration, whereas chronic disease progression is associated with B cell infiltration

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Hair cell counting Phalloidin-labeled hair cells were visualized using a Nikon Eclipse Ti microscope, equipped with a Yokogawa CSU-W1 spinning, confocal unit (Nikon AG Instruments, Egg, Switzerland), and a Photometrics Prime 95B camera, using NIS (version 5.21)