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After 24 h of incubation, the cells are lysed directly in the wells following the protocol described on the RNeasy Mini kit (Qiagen) according to the manufacturer's protocol

To determine whether the colistin-induced depolarization was the result of the opening of the mitochondrial permeability transition pore (mPTP), mitochondria were co-incubated with colistin and cyclosporin A, a well-characterized desensitizer of the mPTP 31,32

The average per cent change in hair loss was calculated in the following way: The per cent change in hair loss from the beginning of the study to its end was determined for each study subject

doi: 10.3390/vetsci8110244

The Shannon index takes into account the richness (i.e., amount of taxonomic groups)