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Huh-7 cells were then transfected using the Cas9-sgRNA complex and DNA was extracted using QuickExtract DNA Extraction

American Journal of Nephrology

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Analysis scripts and notebooks used for this study are open-source and available from GitHub ( Any additional information required to reanalyze the data reported in this work paper is described in the Methods and available upon request from the corresponding authors

To investigate this, we pharmacologically inhibited the cystine/glutamate transporter in the model of TLS