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The dissolved oxygen concentrations in bottom water were higher in May and June than in July

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After culture for 6 h at 20C, cells were harvested by centrifugation (15 min, 6000 g, 4C), re-suspended in PBS (15 ml per pellet from 1 L of culture), and subsequently lysed by sonication (cycle of 30 0.5 s pulses at a power output of 70% per pellet from 1 L of culture) using a Branson Sonifier Digital 450 (Branson Ultrasonics, Danbury, CT, USA)

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