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Additionally, the ethane group linker and the amino group of -alanine form the same interaction as that of taurine with hTauT: the two-carbon linker is nested into the subsites C lined by the hydrophobic side chain of L134 and Y138 on TM2, F300, A303, and L306 on TM6, and the amino group of -alanine also forms a hydrogen bond with the side chain of E406 on TM8 and water-mediated interactions with the main chain carboxylate group of F300 on TM6 and the side chain of Q403 on TM8 (Fig

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Im not sure how he did that