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The following FBXO3 crRNA guides were ordered from IDT and used: sgFBXO3-3: GGAGGATTCCAGCAGAGACA sgFBXO3-4: TATCAAGTCATGATCCGCTG sgFBXO3-5: TGTTCATACCGAATTCACAA sgFBXO3-6: GACGTCGATACAGCTGCCGG Endpoint HiBiT protein degradation HiBiT reporter cells, generated as described above, were seeded into 384-well plates at 20,000 cells per well and treated with the indicated compounds and equivalent concentrations of DMSO for the time indicated in the experiment, usually 3, 24 or 72 h

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Together, these two procedures enable quantification of the total H 2 S, terminal S atoms in RSS and both terminal and internal S atoms in RS(S) n S