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Description

Pharmacol Ther 2011;132:31432

Hummers WS, Offeman RE

Minimizing side effects Several strategies reduce the likelihood and severity of side effects: Start low and titrate up

Foxp3 has been reported to suppress PI3K-AKT-mediated Glut1 upregulation and the glycolytic program through to maintain suppressive capacity and survival in low glucose environments

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Immediately after isolation, CD8 + T cells were activated with anti-human CD3/CD28 Dynabeads (Thermo Fisher Scientific, 11131D) at a 1:1 cell:bead ratio and cultured in complete RPMI 1640 supplemented with 10% FBS, 100 U ml 1 penicillin, 100 g ml 1 streptomycin and 30 U ml 1 IL-2 (Sigma-Aldrich, 11147528001)