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In addition to its anti-apoptotic effects, PCA exerts anti-tumor activity through various mechanisms

After washes, cells were incubated with AlexaFluor-labeled secondary antibodies and Alexa488- or Alexa647-labeled phalloidin (Invitrogen, diluted at 1:500) diluted in 0.1% (wt/vol) BSA-containing TBS for 1 h at room temperature

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To compare the metabolic prioritization in restoring levels of glutamate and GABA after ischemia, we calculated the Glu/GABA ratio, which was increased over the first 72 h reperfusion time in all ischemic brain substructures (Fig