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The kidneys high oxygen demand for blood filtration renders it exquisitely sensitive to redox imbalancean aberration common to both CKD and acute kidney injury (AKI) that, when coupled with iron dysregulation, unleashes ferroptosis: a non-apoptotic, iron-dependent form of regulated cell death driven by iron accumulation, lipid peroxidation, and antioxidant defense impairment (e.g., GPX4/SLC7A11 dysfunction), cascades to which the redox-sensitive kidney is uniquely predisposed

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Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to BSA

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10.1016/j.yfrne.2019.100815 209 JakA

[DOI] [PMC free article] [PubMed] [Google Scholar] 24.Hazan S., Stollman N., Bozkurt H.S., Dave S., Papoutsis A.J., Daniels J., Barrows B.D., Quigley E.M., Borody T.J