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They have considerable potential for clinical applications, such as treating bacterial infections, tumors, and neuromuscular diseases

Burrowing as a non-reflex behavioural readout for analgesic action in a rat model of sub-chronic knee joint inflammation

However, for the lysine peptide assay there are two differences between the conditions used by these authors and our method

On one hand, the ability of a compound to react with cysteine has been linked to the development of skin sensitization and is the foundation of KeratinoSens, another ECVAM approved in vitro method developed to replace animal testing

Huang et al., 2021

Samples were filtered through cell strainer tubes and analysed by a FACS Canto II system equipped with 405-nm, 448-nm and 633-nm laser lines