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Molecular basis for familial isolated growth hormone deficiency

Separated proteins were transferred onto Immobilon-P Transfer membranes (MilliporeSigma, IPVH00010), blocked for 1 h using 5% BSA in TBS0.5% Tween-20 and stained overnight with primary rabbit-anti-GCLC (Sigma-Aldrich, HPA036359) or affinity-purified rabbit-anti-GGT1 (GGT129, as previously described 63 ) 1:1,000 in BSA 2.5% overnight at 4 C

Format: Pre-formulated Aerosol spray Total Volume: 10ML Preparation: Ready-to-use research format This product arrives pre-formulated in a Aerosol spray bottle for research use

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