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More specifically, sCD14 was independently associated with TMAO in untreated HIV-infected subjects (Haissman et al., 2017)

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7 L of RNA was added to the RT and buffer mixture prepared with random hexamers and oligoDT primers as per kit instructions, and rtPCR carried out with the following programme: 10 min at 25C, 30 min at 55C and 5 min at 85C