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For homologous recombination the CEN and URA fragments along with the SEO1 gene fragment, carrying vector overlapping sequences, were transformed in yeast using lithium acetate method and plated on SD-URA plates

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Although the daily requirement of vitamin B 12 is approximately 2 mcg, the initial oral replacement dosage consists of a single daily dose of 1,000 to 2,000 mcg (Table 4)

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So you bring up the evidence-based, uh, data at the same time as youre-youre talking about things

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