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Superoxide is rapidly removed by high concentrations of scavenging enzymes called superoxide dismutases (SOD) with distinct isoenzymes located in the mitochondria, cytoplasm, and extracellular compartments

1 Introduction Aging manifests in multiple systems throughout the entire body

Initially (untreated cells for initial time point) or after 4 days (with varying treatment conditions), 40 L of Cell Titer Glo reagent (Promega) was added to each well, mixed briefly, and the luminescence read on a luminometer (Molecular Devices)

2016, Accessed 18 Aug

These chemical agents are preferred due to their ability to directly damage the urothelium and other bladder cells, leading to mucosa erosion, hemorrhage, edema, and leukocytic infiltration of the bladder wall, which closely resembles the pathology seen in human IC/BPS cases

Korean Association for the Study of Intestinal Diseases