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The emerging role of ferroptosis in female reproductive disorders

Antioxidants Many biochemical reactions in the human body are enzyme catalyzed reactions, most of these enzymes with thiol as active groups, the state of the thiol group determines the activation and inhibition of enzyme activity

doi: 10.1016/S0074-7742(07)82016-2

The GAPDH gene was served as the internal control ( CT method ( Cloning, expression and identification of rSmGST The first Spirometra GST gene deposited in GeneBank (AEI16476.1) was amplified by PCR with specific primers carrying BamHI and PstI restriction enzyme sites (underlined) (forward, 5-AT GGATCC ATGGGTTC GCTCCCGGTTC-3, and reverse, 5-AT CTGCAG CTAAGCATCACCACGCCAG-3), and the cycling protocol was as follows: 30 cycles of 95C for 50 s, 60C for 50 s and 72C for 50 s

Nuclear factor (erythroid-derived 2)-like 2 and thioredoxin-1 in atherosclerosis and ischemia/reperfusion injury in the heart

The primer sequences used were as Table 1 follows: Table 1 List of primers used for RT-PCR