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Homogenised tissue was filtered through a 40 m sieve (Corning, Massachusetts, United States), centrifuged at 500 g for 5 min at 4 C to pellet the nuclei and resuspended in buffer supplemented with 1% BSA and 0.2 U/l RNAase inhibitor (Takara Bio, Kusatsu, Japan)

The glucagon-like peptide-1 (GLP-1) receptor agonist semaglutide and the dual agonist for GLP-1 and glucose-dependent insulinotropic polypeptide (GIP) receptors tirzepatide demonstrate remarkable efficacy for diabetes remission and metabolic restoration [90, 91]

Factors such as skin type, underlying health conditions, and current medications must be thoroughly assessed to ensure safety and efficacy

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Scorletti E, Byrne C (2013) Omega-3 fatty acids, hepatic lipid metabolism, and nonalcoholic fatty liver disease

Sperandio S, Poksay K, de Belle I, Lafuente MJ, Liu B, Nasir J, et al