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Immunofluorescence confirmed that the ER-tag colocalizes with ER marker calnexin (Fig

Meanwhile, the cells following the same treatment were harvested, processed into single-cell suspensions, and subjected to FCM analysis to quantify the level of intracellular NO, ROS, and ONOO -

Notably, a recent study found that LAT1 was a pH-dependent but not pH-independent transporter, whose maximal transporter activity is at neutral pH but much lower at acidic pH (Cosco et al., SLC7A5 ) imports BCAAs and several essential amino acids (e.g., phenylalanine, leucine, isoleucine, tryptophan, histidine, and tyrosine) with high affinity in exchange for the efflux of intracellular histidine, tyrosine, and glutamine (Nicklin et al., 2009

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