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However, -PL synthesis is a complex process, involving cell growth, precursor biosynthesis, energy metabolic, redox equilibria, and transportation of substrates and products, making the metabolic pathway of -PL is strictly regulated by complicated global regulatory systems

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Preparation Condition 4 (EDTA 4 o C deproteinization): Storage temperature affects total GSH, free GSH, and GSSG, but not the ratio of free GSH to GSSG in plasma We included EDTA-treated plasma stored at 4 o C (Preparation Condition 4) as a positive control for enzymatic degradation of glutathione which may occur during sample storage

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glyceraldehyde-3-phosphate dehydrogenase, phosphoglycerate kinase, enolase), chaperones (e.g

Add 200 mL of 0.9% Sodium Chloride injection to the vial using the transfer spike